596 research outputs found
Experiment Study of a Water Injected Twin Screw Compressor for Mechanical Vapor Compression System
The mechanical vapor compression (MVC) system is a promising way for energy saving among energy intensive industries such as desalination, food and beverage, chemistry and waste water concentration etc. In practical its application is limited by the vapor compressor technology which is demanded to have high pressure ratio for large saturation temperature difference and low discharge steam temperature. Due to the ability of wet compression, twin screw compressor can overcome the technical limitation above by the injection of liquid water into the working chamber. In this paper a test rig of a MVC system with water injected twin screw compressor has been designed and built. Compressor performance of flow rate, power consumption and discharge temperature was measured. Moreover, operation characteristics of the water injected twin screw compressor were studied by the measurement of its working process p-V diagram. Volumetric efficiency and adiabatic efficiency of the compressor variation under different operation conditions were calculated. The mass flow rate and temperature of injected water was also measured and adjusted for optimization. The results of the experiment study can provide some design guidances of water injected twin screw compressor for mechanical vapor compression system
Polycystin-1 inhibits eIF2α phosphorylation and cell apoptosis through a PKR-eIF2α pathway.
Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in PKD1 or PKD2 which encodes polycystin-1 (PC1) and polycystin-2, respectively. PC1 was previously shown to slow cell proliferation and inhibit apoptosis but the underlying mechanisms remain elusive or controversial. Here we showed in cultured mammalian cells and Pkd1 knockout mouse kidney epithelial cells that PC1 and its truncation mutant comprising the last five transmembrane segments and the intracellular C-terminus (PC1-5TMC) down-regulate the phosphorylation of protein kinase R (PKR) and its substrate eukaryotic translation initiation factor 2 alpha (eIF2α). PKR is known to be activated by interferons and dsRNAs, inhibits protein synthesis and induces apoptosis. By co-immunoprecipitation experiments we found that PC1 truncation mutants associate with PKR, or with PKR and its activator PACT. Further experiments showed that PC1 and PC1-5TMC reduce phosphorylation of eIF2α through inhibiting PKR phosphorylation. Our TUNEL experiments using tunicamycin, an apoptosis inducer, and GADD34, an inhibitor of eIF2α phosphorylation, demonstrated that PC1-5TMC inhibits apoptosis of HEK293T cells in a PKR-eIF2α-dependent manner, with concurrent up- and down-regulation of Bcl-2 and Bax, respectively, revealed by Western blotting. Involvement of PC1-regulated eIF2α phosphorylation and a PKR-eIF2α pathway in cell apoptosis may be an important part of the mechanism underlying ADPKD pathogenesis
Enzyme activity and thermostability of a non-specific nuclease from Yersinia enterocolitica subsp. palearctica by site-directed mutagenesis
Background: To identify the critical amino acid residues that
contribute to the high enzyme activity and good thermostability of
Yersinia enterocolitica subsp. palearctica (Y. NSN), 15 mutants of Y.
NSN were obtained by site-directed mutagenesis in this study. And their
enzyme activity and thermostability were assayed. Effect of several
factors on the enzyme activity and thermostability of Y. NSN, was also
investigated. Results: The results showed that the I203F and D264E
mutants retained approximately 75% and 70% enzyme activity,
respectively, compared to the wild-type enzyme. In addition to the
I203F and D264E mutants, the mutant E202A had an obvious influence on
the thermostability of Y. NSN. According to the analysis of enzyme
activity and thermostability of Y. NSN, we found that Glu202, Ile203
and Asp264 might be the key residues for its high enzyme activity and
good thermostability. Conclusions: Among all factors affecting enzyme
activity and thermostability of Y. NSN, they failed to explain the
experimental results well. One reason might be that the enzyme activity
and thermostability of Y. NSN were affected not only by a single factor
but also by the entire environment
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